Syllabus
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Course Code: MMB-206 Course Name: General Microbiology /MOOCs/Swayam portal |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | History, development, scope and applications of Microbiology. General methods of sterilization. Introduction to Microscopy: bright field microscopy, dark field microscopy, phase contrast microscopy, electron microscopy. Various methods of staining of bacteria (simple, negative and Gram) and fungus (mold and yeast). Whittaker system of classification.Brief introduction to Bergey’s manual and groups of bacteria (gram positive and negative, spirochetes, endosporulating bacteria, actinomycetes, archaea, mycoplasma) |
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| 2 | Morphology & fine structure of bacterial cell wall, cell membrane, flagella and capsules. Formation of spores. Bacterial growth curve and measurement. Nutritional requirements and nutritional types of bacteria. Pure culture techniques –pour plate, spread plate, streak plate and serial dilution agar plate method. Advantages and disadvantages of various techniques. Preservation of microbial culture-serial subculture, at very low temperature, overlaying culture with mineral oil, lyophilization or freeze drying, in liquid nitrogen. |
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