Syllabus
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Course Code: MMB-201 Course Name: Biophysical & Biochemical Techniques |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Microscopy: Basics of microscopy: image formation, magnification, resolution. Wave theory, Electromagnetic theory. Principles and working of bright field microscope, fluorescent microscope, phase contrast microscope, electron microscope (SEM & TEM), dark field microscopy. confocal microscopy. Principles of staining. Flow cytometry- flurochromes, fluorescent probe and working principle and its applications. |
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| 2 | Chromatography: Gel filtration, ion exchange & affinity chromatography, paper chromatography, Thin Layer Chromatography. Basic principles and biological applications of HPLC and GC. Principles and used of MALDI-TOF and LC-MS platforms. Spectroscopy: Basic concepts, principles and biological applications of different types of spectroscopy: UV, IR, NMR, Raman. X-ray diffraction, circular dichromism for microbiologists. |
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| 3 | Centrifugation: Basics of centrifugation based methods: viscosity, diffusion, sedimentation equilibrium, dialysis, solvent fractionation, centrifugation, Biological applications and interpretations of Density Gradient methods, Ultracentrifugation methods. Methods of bacterial and enzyme immobilization, their advantages and applications. Basics of Radioactive isotopes and radioactive decay, sample preparation, counting, Safety precautions during handling, biological applications. |
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| 4 | PAGE: Polyacrylamide gel electrophoresis (PAGE), native PAGE, SDS-PAGE, 2D electrophoresis, iso electric. Types of Agarose gel electrophoresis. Protein engineering and proteome analysis: Proteome analysis by 2D gel electrophoresis coupled to mass spectrometric analysis. PMF verses MS/MS. Protein arrays and their applications. DNA Microarray and its applications. |
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