Syllabus
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Course Code: BCH-406 Course Name: Practical-08 (Based on papers BCH-403 and BCH-404A&B) |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
|---|---|---|
| 1 | To study some of the routinely used equipments in microbiology laboratory | |
| 2 | Storage of microorganisms | |
| 3 | Preparation of solid and liquid media for growth of microorganisms | |
| 4 | Isolation of bacteria from soil and maintenance of microorganisms by plating, streaking and serial dilution method | |
| 5 | To perform slant and stab culture | |
| 6 | To perform Gram staining in order to differentiate between gram positive and gram negative bacteria | |
| 7 | To demonstrate bacterial transformation | |
| 8 | Separation of poly RNA on oligo dT column | |
| 9 | To perform agarose gel electrophoresis | |
| 10 | Determination of molecular weight of DNA fragments on agarose gel electrophoresis | |
| 11 | To perform PCR /RFLP using restriction enzymes | |
| 12 | To demonstrate Southern blotting and Northern blotting | |
