KUK - LMS

Syllabus

MODULE NO / UNIT	COURSE SYLLABUS CONTENTS OF MODULE	NOTES
Course Code: BCH-403 Course Name: Genetic Engineering
1	Gene cloning strategies: Isolation and purification of nucleic acid and its quantification and analysis; Molecular tools and their applications; Restriction endonucleases; DNA modification enzymes; Site directed mutagenesis; Cloning vectors; Ligation of DNA fragments: Linkers, adapters and homopolymeric tailing; Construction of genomic library: mRNA enrichment; Reverse transcription; Synthesis of cDNA and library construction.
2	Expression vectors: Choice of expression system; Expression in bacterial, yeast, insect and mammalian cells; Baculovirus expression systems; Expression of heterologous genes; Factors affecting the expression of cloned genes; Codon bias; Vector engineering and codon optimization; Transgenic and gene knockout technologies: Transgenic methodology; Transgenic animals and plants; Targeted gene replacement; chromosome engineering.
3	Studying gene expression and function: Studying the transcript of a cloned gene; Identifying protein binding sites on a DNA molecule; Identifying control sequences by deletion analysis; Identifying and studying the translation product of a cloned gene by HRT & HART. Studying protein-protein interactions (Phage display and the yeast two hybrid systems). Production of Proteins from cloned genes: Expression in E. coli (Vectors for expression of foreign genes in E. coli, promoters used in expression vectors, general problems with the production of recombinant protein in E. coli);Production of recombinant protein by eukaryotic cells (Recombinant protein production in yeast, insect cells and mammalian cells; Pharming- recombinant protein production from live animals and plants); Recombinant protein purification using His-tag. Importance of gene cloning in medicine for the production of recombinant pharmaceuticals
4	Intellectual Property Rights: Introduction to IPR, Types of IPR - Patents, Trademarks, Copyright and Related Rights, Industrial Design, Traditional Knowledge and Geographical Indications. Importance of IPR - patentable and nonpatentable, IPR and WTO regime - consumer protectionand plant genetics resources. Bioethics: Introduction to ethics and bioethics; Ethical and socioeconomic aspects of gene therapy, germline, somatic, embryonic and adult stem cell research. Ethical implications of GM crops, GMOs, human genome project, human cloning and bio-weapons.