Syllabus
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Course Code: BCH-402 Course Name: Biotechniques |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Radioisotope techniques: Basic concepts (types of radioactive decay, rate of radioactive decay, radioactive isotopes and their half-lives and units of radioactivity); GM and scintillation counter; autoradiography; specific activity of a radioisotope; safety aspects; applications of radioisotopes in biological sciences. Centrifugation: Basic principles; different types of centrifuges; types of rotor; analytical and preparative ultracentrifugation methods. |
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| 2 | Molecular biology techniques: Isolation of DNA and RNA, purification and quantification of nucleic acids;Electrophoresis of nucleic acids: agarose gel electrophoresis, pulse field electrophoresis; capillary electrophoresis; microchip electrophoresis; DNA sequence analysis methods: Sanger dideoxy method, Maxam Gilbert chemical method and Fluorescence method; Polymerase chain reaction: principles, process, design and optimization; different types of PCR: allele specific, nested, multiplex and real-time PCR; ligase chain reaction; SNP and application in molecular diagnostics; DNA fingerprinting: applications and prospects; restriction fragment length polymorphism (RFLP) and its uses. | |
| 3 | Immunotechniques: Immunoprecipitation; agglutination; RIA; ELISA; ELISPOT; immunoblotting; immunofluorescence assays; cytotoxic assay; hybridoma technology for production of monoclonal antibody - principles, techniques and applications; designing chimeric and humanized antibodies; vaccines: types and their role in prevention of diseases. | |
| 4 | Spectroscopy: Nature of electromagnetic radiations; principles of biophysical methods used for analysis of biopolymer structure - UV, Visible, Infrared, Raman, Fluorescence and NMR spectroscopy; ORD and CD; Atomic absorption spectroscopy. | |
