Syllabus
|
Course Code: BCH-307 Course Name: Practical-5 (Based on papers BCH-301 and BCH-302) |
||
| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
|---|---|---|
| 1 | Extraction of DNA from plant tissue/Human blood and checking of its purity | |
| 2 | Preparation of plasmid DNA | |
| 3 | Agarose gel electrophoresis of DNA | |
| 4 | Isolation of cytoplasmic RNA | |
| 5 | Electrophoresis of RNA on denaturing gels | |
| 6 | Restriction digestion of DNA by restriction endonuclease | |
| 7 | Construction of restriction map of plasmid DNA | |
| 8 | Electrophoretic separation of isoenzymes | |
| 9 | Ligation of DNA fragments | |
| 10 | ELISA | |
| 11 | Immunodiffusion | |
| 12 | Purification of IgG from serum | |
