KUK - LMS

Syllabus

MODULE NO / UNIT	COURSE SYLLABUS CONTENTS OF MODULE	NOTES
Course Code: BCH-204 Course Name: Molecular Biology -I
1	Basic Concepts of Genetic Information: Nucleic acids as the genetic material - experimental evidences; Chargaff’s rules Structure of DNA, Structural polymorphism of DNA (A, B and Z-DNA) various forces responsible for stability of DNA, DNA topology, topological and geometric properties, DNA supercoiling, Topoisomerases in prokaryotes and eukaryotes, DNA organization in prokaryotes and eukaryotes, C-value paradox, denaturation: different ways for carrying out denaturation, renaturation: requirements, kinetics, significance, various classes of DNA: highly repetitive, moderately repetitive and unique sequence, RNA: structure and types.
2	DNA replication, mutations and DNA repair: Possible modes of DNA replication, Meselson-Stahl experiment, DNA polymerases and other enzymes involved in DNA replication, Okazaki fragments, Mechanism of replication in prokaryotes and eukaryotes, inhibitors of DNA replication, molecular basis of mutations, DNA repair mechanisms like direct, base-excision, nucleotide-excision, mismatch, SOS and recombinational repair.
3	Transcription and post-transcriptional modifications: RNA polymerase/s in prokaryotes and eukaryotes, DNA footprinting technique, initiation, elongation and termination of transcription in prokaryotes and eukaryotes, inhibitors of transcription, RNA replicase, reverse transcriptase, post-transcriptional modifications: different types of introns and their splicing mechanisms, processing of mRNA, rRNA and tRNA precursors, overlapping genes and split genes.
4	Protein synthesis, targeting and degradation: Characteristics of the genetic code, biological significance of degeneracy, decoding the code, Wobble hypothesis, ribosomes structure and function in prokaryotes and eukaryotes, Aminoacyl tRNA-synthetases, various factors and steps involved in protein synthesis in prokaryotes and eukaryotes, polyribosomes, post-translational processing, signal hypothesis and protein targeting to lysosomes, Plasma membrane, extracellular matrix and different compartment of mitochondria and chloroplast, protein degradation.