Syllabus

Course Code: BCH-303    Course Name: Plant Biochemistry

MODULE NO / UNIT COURSE SYLLABUS CONTENTS OF MODULE NOTES
1 Chemical and physical composition of higher plant cell wall.
Light reactions of Photosynthesis: Photosynthetic pigments, chlorophyll excitation by absorption of light energy and its return to the ground state, Requirement of an antenna to capture light, van Niel equation, Hill equation, Cyclic electron transport in purple photosynthetic bacterium, Red drop and Emerson enhancement effect, Photosystem I & II, Non-cyclic, cyclic and pseudocyclic photosynthetic electron transport, Inhibitors of non-cyclic electron transport, Regulation of energy distribution between PS I and PS II, Photophosphorylation: coupling between electron transport and phosphorylation, chemiosmotic hypothesis, chloroplast ATP synthase, binding change mechanism of ATP synthesis and uncouplers of photophosphorylation.
2 Pathway and regulation of CO2 assimilation in C3, C4 & CAM plants. Photorespiration: pathway and significance. Metabolism of Sucrose and Starch: Biosynthesis and degradation of starch and sucrose; role of fructose 2, 6- bisphosphate in carbon partitioning between sucrose and starch.
Electron transport in plant mitochondria: Electron transport complexes and pathway of electron flow in plant mitochondria; cyanide - resistant respiratory pathway.
3 Nitrogen Metabolism: Nitrogen Cycle; Nitrate Assimilation: nitrate uptake, nitrate & nitrite reduction and regulation of nitrate assimilation.
Biological nitrogen fixation: Nitrogen fixing organisms, structure and mechanism of action of nitrogenase, Legume- Rhizobiumsymbiosis(Abriefaccount),Leghaemoglobin,Strategiesforprotectionofnitrogenaseagainsttheinhibitory effect of oxygen, Uptake hydrogenase, Ammonia assimilation, nifgenes of Klebsiella pneumoniae and their regulation, and synthesisofamidesandureides.
Sulphateassimilation:sulphate up take and its assimilation into cysteine.
4 Biochemical defense mechanisms in plants against pathogens; Plant hormones: Physiological effects and molecular mechanism of action of auxins, gibberellins, cytokinins, ABA and ethylene. Phytochromes as light sensors.
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