KUK - LMS

Syllabus

MODULE NO / UNIT	COURSE SYLLABUS CONTENTS OF MODULE	NOTES
Course Code: BCH-301 Course Name: Molecular Biology -II
1	Gene regulation: Various levels of control of gene expression in prokaryotes and eukaryotes, operon concept, regulation of expression of lac, galactose, araBAD, tryptophan operons and lambda phages, regulation of ribosome synthesis, motifs involved in DNA- protein, protein-protein interactions;Variousregulatory sequences in eukaryotes, molecular aspects of regulation of gene expression at transcription level viz. repression by nucleosomes, DNase sensitivity and hypersensitivity, histone modifications etc., at post-transcriptional level like regulation of RNA splicing, RNA transport, RNA stability; at translational, post-translational and protein degradation level.
2	Transposable genetic elements: Non-replicative and replicative transposition, transposable genetic elements in bacteria, yeast, maize, drosophila and significance of transposable elements. Interaction of nucleic acids with small molecules: Reactions of nucleic acids with non-carbon electrophiles, nitrogen electrophiles, carbon electrophiles, anticancer drugs, photochemical modifications of nucleic acids, effects of ionizing radiations on nucleic acids.
3	Molecular Biology of Cancer: Benign and malignant tumors, types of cancers, cancer causing agents- radiations, chemical compounds, DNA and RNA viruses; mechanism of carcinogenesis; important characteristics of cancerous cells; proto-oncogenes and oncogenes, gain of function mutations of proto-oncogenes-growth factors, growth factor receptors, intracellular signal transducers, nuclear transcription factors, cell cycle control proteins, apoptotic proteins, DNA repair proteins into oncogenes; Rb and P53 as tumor suppressor genes, telomerase expression and immortalization of cells.
4	Drosophila development and its regulation: Various stages of oogenesis, blastulation, gastrulation to form three cell layers, morphogen gradient, details of three classes of pattern control genes like egg-polarity genes, segmentation genes, homeotic selector genes and imaginal discs. Genomics: Structural genomics-construction of cytological maps based on banding pattern, physical maps based upon contigs, sequence-tagged sites (STSs), expressed-sequence tags (ESTs), genetic maps based upon RFLP, microsatellites, variable number tandem repeats; Map position- based cloning of genes; The human genome project; functional genomics- DNA microarray, serial analysis of gene expression (SAGE); comparative genomics- prokaryotic, chloroplast, mitochondria and eukaryotic genomes; evolution of genomes in the cereal grasses and mammals.