Syllabus
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Course Code: BCH-208 Course Name: Practical-4 (Based on papers BCH-203 and BCH-204) |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
|---|---|---|
| 1 | Estimation of DNA by diphenylamine reaction | |
| 2 | Estimation of RNA by orcinol reaction | |
| 3 | Assay of acid phosphatase enzyme from plant/animal tissue and calculation of specific activity | |
| 4 | Assay of alkaline phosphatase enzyme from plant/animal tissue and calculation of specific activity | |
| 5 | Effect of substrate concentration on enzyme activity of acid/alkaline phosphatase | |
| 6 | Effect of enzyme concentration on enzyme activity of acid/alkaline phosphatase | |
| 7 | Effect of temperature and PH on the activity of acid/alkaline phosphatase | |
| 8 | Effect of PH on the activity of acid/alkaline phosphatase | |
| 9 | Determination of Km, and V max | |
| 10 | Determination of PH optima of an enzyme | |
| 11 | Demonstration of enzyme immobilization | |
| 12 | Partial purification of an enzyme by Ammonium Sulphate fractionation | |
