Syllabus
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Course Code: BCH-106 Course Name: Practical-2 (Based on papers BCH-103 and BCH-104) |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
|---|---|---|
| 1 | Titration of a weak acid using a pH meter | |
| 2 | Verification of Beer-Lambert’s law and determination of absorption coefficients | |
| 3 | Concentration of a protein sample by ultrafiltration (using stirred cell) | |
| 4 | Separation of amino acids and carbohydrates in a mixture by Paper chromatography | |
| 5 | Separation of lipids/amino acids by TLC | |
| 6 | Purification of an enzyme by ion-exchange chromatography | |
| 7 | Purification of an enzyme by gel filtration chromatography | |
| 8 | Determination of void volume of a gel filtration column | |
| 9 | Determination of molecular weight of an enzyme by gel filtration | |
| 10 | Separation of proteins by Native PAGE and SDS PAGE | |
| 11 | Determination of molecular weight of a protein by SDS PAGE | |
