Syllabus
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Course Code: BCH-105 Course Name: Practical-1 (Based on papers BCH-101 and BCH-102) |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
|---|---|---|
| 1 | To study biochemistry laboratory safety rules and guidelines | |
| 2 | Preparation of buffers | |
| 3 | Qualitative estimation of carbohydrates | |
| 4 | Qualitative estimation of proteins/amino acids | |
| 5 | Qualitative estimation of lipids | |
| 6 | Quantitative estimation of proteins by Lowry’s method | |
| 7 | Quantitative estimation of proteins by Bradford method | |
| 8 | Quantitative estimation of total sugars | |
| 9 | Quantitative estimation of reducing sugars by Nelson-Somoyogi’s method | |
| 10 | Solubility test for lipids | |
| 11 | To detect the presence of glycerol in given sample by acrolein method | |
| 12 | Characterization of lipids (Acid value, Saponification value and Iodine number) | |
| 13 | Extraction of lipids from tissues using Soxhlet’s apparatus | |
| 14 | To determine pka of acetic acid/glycine | |
| 15 | Subcellular fractionation of organelles from animal/plant tissue | |
| 16 | To demonstrate: Light microscopy, Fluorescence microscopy, Confocal microscopy, Electron microscopy (scanning and transmission) | |
