Syllabus
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Course Code: M-FSC 204 Course Name: DNA Profiling |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Introduction of Human Genome: Human chromosomes and karyotype, human nuclear genome. Mutation-types and cause, gens and alleles, human genetics and heredity. Calculation of allele frequencies. Basic structure, types and properties of DNA and RNA, mt DNA, DNA modifying enzymes, restriction enzymes. |
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| 2 | Forensic DNA Profiling: History and development of DNA finger printing. Concept of length and sequence variation- VNTRs, STRs, mini STR, SNPs. STR – markers-nomenclature of STR markers. Methods of DNA profiling: Principle, techniques of RFLP, STR profiling their advantage and limitations. Gender identification: Y-STR and mt-DNA profiling. DNA Amplification (PCR)- Principle, method, DNA primers, factors affecting PCR, advantage of PCR based techniques over RFLP. |
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| 3 | DNA sample preparation: Sample sources for DNA, collection and preservation of samples for DNA testing, conventional and recent methods of DNA extraction (for samples blood, tissue, hair, tooth and bones), separation methods, DNA quality check, DNA Quantitation methods, DNA sequencing. DNA data base- CODIS, STRbase. NGS (New Generation Sequecing Rapid DMNA) |
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| 4 | Nucleic acid hybridization: Preparation of nucleic acid probes for DNA profiling, Single locus and multi locus probes, and cDNA probes; Methods of labeling of DNA probes; detection methods. Forensic Issues: Degraded DNA, contamination, mixed samples and low copy number. Result interpretation, Quality assurance in DFP testing. Legal standards for admissibility of DNA profiling Forensic Significance of DNA Profiling: Personal identification, paternity testing, wild life Forensics, veterinary, agriculture and mass disaster. Report writing and presentation of report in case of DNA profiling. |
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