Syllabus
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Course Code: M-FSC 202 Course Name: Instrumental Analysis II |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Molecular Biology Techniques: Isolation of DNA, RNA, purification, restriction enzymes, PCR –DNA amplification, autoradiography, blotting techniques and Forensic utility. Cell and tissue culture techniques: pH and buffers, culture media preparations, sterilization techniques. |
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| 2 | Centrifugation Techniques: Centrifugation, cold and ultracentrifuges basic principle, instrumentation, G-value & relationship between RPM., applications of analytical centrifugation. Electrophoresis: Introduction, principles, factors affecting electrophoresis, types of electrophoresis. High and low voltage electrophoresis, capillary electrophoresis. immuno-electrophoresis, SDS-PAGE and iso- electric focusing; their application. |
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| 3 | Enzyme Techniques: Enzyme kinetics, enzyme assay techniques such as visible UV spectrophotometric methods, Luminescence method, Radioisotope methods and Immuno-chemical methods. Radio-chemical Techniques: Radioisotope, nature of radioactivity, detection and measurements of radioactivity and applications. |
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| 4 | Immunochemical Techniques: Introduction, Antigen - antibody reactions - theory and principles, Production of antibodies. Immunoprecipitation and agglutination based techniques such as immunodiffusion, cross over electrophoresis etc. Labeling of Antibodies and their detection methods: ELISA, RIA- their basic principle, techniques and their Forensic applications |
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