Syllabus
Course Code: M-Z 408 (i) Elective Course Name: Practical (Based on Papers M-Z 404 /Z 405/Z 406) |
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MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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1 | Course Contents (Molecular Reproduction-II): 1. Effects of pesticides, drugs, and xenobiotics on granulosa cells in vitro. |
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2 | 2. Effects of pesticides, oxidants, and free radicals on testicular tissue in vitro. | |
3 | 3. Morphological changes during spermatogenesis. | |
4 | 4. Effects of environmental endocrine disruptors on the fertility after exposure in vitro. | |
5 | 5. Estimation of catalase from reproductive tissues | |
6 | 6. Determination of Glutathione peroxides, glutathione reductase from reproductive tissues. | |
7 | 7. Lipid peroxidation by MDA method. | |
8 | 8. Impact of Vitamin C & E and other antioxidants on pesticides induced cytotoxicity on reproductive tissues in vitro. | |
9 | 9. Isolation of genomic DNA | |
10 | 10. Agarose Gel Electrophoresis: Practical demonstration | |
11 | 11. Age related changes in sperm characteristics. | |
12 | 12. Demonstration of Single Cell Gel electrophoresis/COMET Assay to elucidate toxicological effect. |