Syllabus

Course Code: M-Z 408 (i) Elective    Course Name: Practical (Based on Papers M-Z 404 /Z 405/Z 406)

MODULE NO / UNIT COURSE SYLLABUS CONTENTS OF MODULE NOTES
1 Course Contents (Molecular Reproduction-II):
1. Effects of pesticides, drugs, and xenobiotics on granulosa cells in vitro.
2 2. Effects of pesticides, oxidants, and free radicals on testicular tissue in vitro.
3 3. Morphological changes during spermatogenesis.
4 4. Effects of environmental endocrine disruptors on the fertility after exposure in vitro.
5 5. Estimation of catalase from reproductive tissues
6 6. Determination of Glutathione peroxides, glutathione reductase from reproductive tissues.
7 7. Lipid peroxidation by MDA method.
8 8. Impact of Vitamin C & E and other antioxidants on pesticides induced cytotoxicity on reproductive tissues in vitro.
9 9. Isolation of genomic DNA
10 10. Agarose Gel Electrophoresis: Practical demonstration
11 11. Age related changes in sperm characteristics.
12 12. Demonstration of Single Cell Gel electrophoresis/COMET Assay to elucidate toxicological effect.
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