Syllabus
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Course Code: M-Z 301 Course Name: Molecular Biology |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | 1.0 DNA Replication 1.1 Prokaryotic and Eukaryotic DNA replication 1.2 Mechanics of DNA replication 1.3 Enzymes and accessory proteins involved in DNA replication 2.0 Transcription 2.1 Prokaryotic and Eukaryotic transcription 2.2 General and specific transcription factors 2.3 Regulatory elements and mechanisms of transcription regulation 2.4 Transcriptional and post-transcriptional gene silencing. 2.5 Post-transcriptional Modifications in RNA 2.6 5'-Cap formation, 3'-end processing and polyadenylation 2.7 Splicing, Editing, Nuclear export of mRNA, mRNA stability |
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| 2 | 3.0 Translation 3.1 Genetic code and deciphering of genetic code 3.2 Prokaryotic and Eukaryotic translation 3.3 The translational machinery 3.4 Adaptor hypothesis, Kozak rule 3.5 Mechanisms of initiation, elongation and termination 3.6 Regulation of translation 4.0. Transport of Protein 4.1 Co- and Post-translational transport of proteins 4.2 Co- and Post-translational modifications of proteins 4.3 Protein trafficking/sorting |
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| 3 | 5.0 Recombination and Repair 5.1 Holiday junction, gene targeting, gene disruption 5.2 Cre/lox recombination 5.3 RecA and other recombinases 5.4 DNA repair mechanisms 6.0 Antisense and Ribozyme technology 6.1 Molecular mechanisms of antisense molecules 6.2 Inhibition of splicing, polyadenylation and translation 6.3 Disruption of RNA structure and capping 6.4 Biochemistry of ribozyme; hammerhead, hairpin and other ribozymes 6.5 Strategies for designing ribozymes 6.6 Application of antisense and ribozyme technologies |
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| 4 | 7.0 Molecular mapping of genome 7.1 Genetic and physical maps 7.2 Physical mapping and map-based cloning 7.3 Southern and fluorescence in situ hybridization for genome analysis 7.4 Chromosome micro-dissection and micro-cloning 7.5 Molecular markers in genome analysis RFLP, RAPD and AFLP analysis and their applications 7.6 Molecular markers linked to disease resistance genes 8.0 rDNA Technology: 8.1 Gene-cloning 8.2 Vectors 8.3 cDNA and genomic liberaries 8.4 Blotting techniques 8.5 Chromosome walking 8.6 Application of rDNA technology |
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