Syllabus

Course Code: M-Z 310    Course Name: Practical (Based on Papers M-Z 304/Z 305/Z 306)

MODULE NO / UNIT COURSE SYLLABUS CONTENTS OF MODULE NOTES
1 1. Demonstration of male and female reproductive systems of earthworm, grass hopper, and rat.
2 2. Processing of reproductive tissues for microanatomy and histochemistry.
3 3. Study of permanent slides on mammalian reproductive tissues.
4 4. Study of folliculogenesis and atresia in rat ovary.
5 5. Study of apoptosis in gonads.
6 6. Extraction and estimation of macromolecules such as proteins, carbohydrates, lipids, and nucleic acids.
7 7. Isolation of testicular cells and ovarian follicular cells.
8 8. Oocyte collection; aspiration and slicing method.
9 9. Sperm morphology, capacitation, sperm count, and sperm motility.
10 10. Vaginal smear preparation to examine estrous cycle and pregnancy.
11 11. Oocyte maturation in vitro.
12 12. In vitro fertilization
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