Syllabus
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Course Code: M-Z 105 Course Name: Practical (Based on Papers M-Z 101 & M- Z 102) |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
|---|---|---|
| 1 | Course Contents: 1. Preparation of mitotic chromosomes from onion root tips |
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| 2 | 2. Preparation of meiotic chromosomes from grasshopper testes. | |
| 3 | 3. Preparation of karyotypes from micrographs. | |
| 4 | 4. Calculation of morphometric data and preparations of idiogram. | |
| 5 | 5. Determination of chiasma frequency and terminalization coefficient. | |
| 6 | 6. Study of permanent slides of different stages of meiosis and mitosis. | |
| 7 | Preparation of polytene chromosomes (Chironomous/mosquito) and mapping. | |
| 8 | 8. Preparation of Solutions- Standard solution, Molar, Molal and Normal solution. | |
| 9 | 9. Proteins: Quantitative estimation of proteins by Biuret method and Lowry’s method. | |
| 10 | 10. Carbohydrates: quantitative estimation of total carbohydrates and glucose | |
| 11 | 11. Analysis of Fats/ Oils: iodine number, saponification value, acid value quantitative estimation of total lipids. | |
| 12 | 12. Preparation of Standard curve for the estimation andextraction of nucleic acids (DNA and RNA). | |
| 13 | 13. Paper chromatography: amino acids and carbohydrates. | |
| 14 | 14. Thin layer chromatography: neutral and phospholipids. | |
| 15 | 15. Tools: demonstration of parts and working of the following tools: PCR, GLC, Spectrophotometers, various kinds of microscopes, pH meter, Electrophoresis, Centrifuges, Tissue culture unit, Incubators | |
| 16 | 16. Microbiological media preparation, sterilization, dilution, inoculation and standard plate count. | |
