Syllabus

Course Code: M-Z 207    Course Name: Practical (Based on Papers M-Z 201 & M- Z 202)

MODULE NO / UNIT COURSE SYLLABUS CONTENTS OF MODULE NOTES
1 Course Contents:
1. Study of various components of pond and grassland ecosystem.
2 2. Determination of Water quality characteristics viz: Dissolved oxygen, pH, free carbon dioxide, salinity, transparency, alkalinity and hardness.
3 3. Methods of population density measurements.
4 4. Estimation of biodiversity indices.
5 5. Field Visit to Aquatic, Forest and other ecosystems for identification of biota.
6 6. Preparation of tissues for microtomy and demonstration of cryo techniques
7 7. Histochemistry: Methods of fixation of different tissues.
8 8. Histochemical test:
(a) Haemotoxylin-eosin
(b) Toluidine Blue
(c) Sudan Block-B
(d) Mercury bromophenol blue
(e) Methyl green-pyronin-Y
(f) Periodic acid Schiff’s
(g) Acid phosphatase
(h) Alkaline phosphatase
9 9. Demonstration of live gametes and their staining procedure.
10 10. Determination of optimum pH, temperature and concentration for optimum activityof salivary amylase,
11 To demonstrate that the optimum activity of trypsin enzyme is pH temperaturedependent.
12 12. Qualitative test of vitamins and Quantification of vitamin A and C.
13 13. Total RBC, WBC and Different WBC count
14 14. Estimation of Blood plasma
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