Syllabus
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Course Code: M-Z 102 Course Name: Biochemistry and Bio-techniques |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | 1.0 General Principles of Biochemistry and chemical composition of life 1.1 General Introduction to Biomolecules 2.0 Protein Biology: 2.1 Primary, Secondary, tertiary and quaternary structure of proteins: i)Domain, ii)Reverse turn of Ramachandran plot and its significance 3.0 Enzyme: 3.1 Classification and nomenclature, 3.2 Co-enzymes and Cofactors 3.3 Induced fit and Molecular Mechanism of Enzyme action, 3.4 Enzyme feedback mechanism, Isozymes. 4.0 Nucleic acids: Structure and Functions 4.1 DNA structure and functions 4.2 RNA structure and functions, 4.3 DNA choreography 4.4 Qualitative and quantitative estimation of DNA |
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| 2 | Metabolism: 5.1Glycolysis, citric acid cycles its regulation and role as metabolic hub. 5.2 Hexose monophosphate pathway its regulation and significance. 5.3 Cholesterol biosynthesis, its metabolism and steroidogenesis. 5.4 Bile acids and their metabolism 5.5 Saturated and unsaturated fatty acid and their metabolism. |
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| 3 | 6.0 Chemical and Biological assays (in vitro and in vivo assays). 7.0 Principles and uses of analytical instruments: 7.1 Microscopes and imaging 7.2 Spectrophotometers, 7.3 NMR spectrophotometer 8.0 Microbiological and cell culture Techniques 8.1Setting of microbiological laboratory, 8.2Sterilization and Media preparation techniques 8.3 Inoculation and growth monitoring (Standard plate count technique), 8.4Isolation of a microbial colony and slant preparation. 8.5Design and functioning of tissue culture laboratory, 8.6Basics of cell/tissue culture, Culture media preparation, 8.7Cell proliferation measurements 8.8Cell viability testing and Cell harvesting methods. 8.9 Biosafety and levels 9.0Cryotechniques : 9.1Cryopreservation for cells, tissue, organisms, 9.2Cryotechniques for microscopy. |
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| 4 | 10.0Separation techniques in biology. 10.1 Molecular separations by chromatography, electrophoresis, precipitation etc. 10.2 Organelle separation by centrifugation. Density gradient centrifugation, Ultra Centrifugation, unit gravity centrifugation, affinity adsorption, anchorage based techniques etc. 10.3 Cell separation by flow cytometery and FACS 11.0 Radioisotope and mass isotope techniques in biology: 11.1 Sample preparation for radioactive counting 11.2 Autoradiography 11.3 Biosensors 12.0 DNA fingerprinting |
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