Syllabus
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Course Code: BOT-403 Course Name: Plant Tissue Culture |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | 1. History of Plant Tissue Culture, Basic concept, principles and scope of plant cell and tissue culture, concepts of cellular differentiation; Totipotency; basic techniques of plant tissue culture; callus formation, organogenesis and embryogenesis. 2. Protoplast isolation, fusion and culture, somatic hybridization, hybrid selection and regeneration. Cybrids and their application. |
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| 2 | 3. In vitro haploid production and its significance, Anther/Pollen culture and ovary culture; Embryo and ovule culture Production of triploids through endosperm culture. 4. Micropropagation: meristem culture and virus-free plants; Cryopreservation of plant cell and tissue cultures and establishment of gene banks. |
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| 3 | 5. Somaclonal variations and isolation of useful mutants; mechanisms and applications in genotype improvement. 6. Role of plant cell cultures in Bioreactor types and application in cell culture and secondary metabolite production. |
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| 4 | 7. Somatic embryogenesis, production of synthetic seeds, importance, limitation and their utilization. 8. Application of tissue culture in forestry and agriculture; status of tissue and cell culture technology in India edible vaccines, and their prospects |
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