Syllabus
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Course Code: BOT-304 Course Name: e) Biophysical & biochemical techniques (elective) |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | 1. Microscopic techniques: Introduction; Light microscope; Phase contrast microscope; Fluorescent microscope; Electron microscope (EM) – SEM, TEM and STEHM; Scanning probe microscopes- scanning itrogeni microscope and atomic force microscope; Different fixation and staining techniques. 2. Centrifugation: Principles of sedimentation; Types, care and safety aspects of centrifuges; Differential centrifugation; Density gradient centrifugation and their applications. |
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| 2 | 3. Chromatographic techniques: Theory of chromatography; Types of chromatography- Paper chromatography, Thin layer chromatography, Adsorption chromatography, Partition chromatography, Affinity chromatography, Ion exchange chromatography, HPLC and Size-exclusion chromatography. 4. Spectrophotometery: Colorimetery; UV and Visible spectrophotometery. |
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| 3 | 5. Electrophoresis: Principle; Agarose gel electrophoresis; Polyacrylamide gel electrophoresis; 2-Dimensional gel electrophoresis; Capillary electrophoresis; Microchip electrophoresis and Isoelectric focusing. 6. Mass spectrometry: Introduction; Theory; Mass spectrometer; Ionization of molecules; Mass analysers- MALDI; Detectors and Applications. |
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| 4 | 7. Immunotechniques: Antibody generation; Detection of molecules using ELISA, RIA, Immunoprecipitation and Immunofluorescence microscopy; Detection of molecules in living cells. 8. Radioisotope techniques: Radioactive isotopes; Nature of radioactivity; Detection and measurement of different types of radioisotopes normally used in biology; Incorporation of radioisotopes in biological tissues and cells; Molecular imaging of radioactive material; Disposable of radioactive wastes and safety guidelines. |
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