Syllabus
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Course Code: BOT-204 Course Name: Molecular genetics |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | 1. Eukaryotic genome: Different forms of DNA, C- value paradox, unique and repetitive DNA, gene families, hybridization kinetics and split genes. 2. Transposable elements: Mechanisms of transposition; transposons in bacteria, maize, Drosophila and yeast. 3. DNA Replication: Semi-conservative, bidirectional, replication origins, replication machinery. |
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| 2 | 4. Mutations: types, isolation of mutants, molecular basis of mutations. 5. DNA damage and repair: Causes of DNA damage; Photoreactivation, excision, mismatch, post replication and error prone repair systems. 6. Fine structure of gene: cis-trans test, rII locus, fine structure analysis of eukaryotes. 7. Bacterial genetics: conjugation, transduction and transformation. |
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| 3 | 8. Transcription: Initiation, elongation and termination in prokaryotes and eukaryotes, RNA polymerases. 9. RNA Processing: Processing of mRNA, rRNA and tRNA. 10. Genetic code: Deciphering the genetic code, characteristics. 11. Translation: Initiation, elongation and termination in prokaryotes and eukaryotes. |
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| 4 | 12. Regulation of gene expression in prokaryotes: Operon concept, lac operon regulation by positive and negative mechanism, trp operon, regulation by negative and attenuation. 13. Regulation of gene expression in eukaryotes: a) Transcriptional level – Regulatory sequences, nucleosome positioning, chromatin remodelling, histone modifications. b) Post-transcriptional level – RNA splicing, RNA stability. c) Translational level and post-translational level. |
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